The Globe, which he won in 1970 for Best Actor, is one of more than 700 items of Wayne memorabilia that include cowboy hats, costumes and saddles.
Among the items in the collection are the iconic black eye-patch Wayne wore in True Grit and the cowboy hat he wore in the1975 sequel, Rooster Cogburn.
The auction will take place at the Hyatt Regency Century Plaza Hotel in Los Angeles and online, Oct. 6-7, 2011.
"My father's fans were very important to him. He was open and accessible to them, and making these items accessible to the public is something that feels right," said Ethan Wayne, President of John Wayne Enterprises. "Museums have large collections of my father's personal property, and our family has had a chance to select and keep items sentimental to us. There is no need to keep this memorabilia locked away when it can be enjoyed by his fans."